Doctor Octopus could refer to:
- Otto Octavius, A Marvel Comics supervillain and foe of Spider-Man.
- Doctor Octopus, Version of Doctor Octopus from the 1967 Spider-Man animated series.
- Doctor Octopus, Version of Doctor Octopus from the 1982 Incredible Hulk animated series.
- Doctor Octopus, Version of Doctor Octopus from the 1994 Spider-Man animated series.
- Carolyn Trainer, Also known as Lady Octopus, Otto's successor.